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10.1002/ajmg.a.35796 50 HorieN.AibaH.OguroK.HojoH.TakeishiK

Transmission electron microscopy and BODIPY staining Samples collected from each infection stage were fixed in 2.5% ( v/v ) glutaraldehyde at 4 C overnight, and were washed with 0.1 M phosphate buffer saline (PBS), post-fixed in 1% ( w/v ) osmic acid for 2 h, washed again with 0.1 M PBS, dehydrated sequentially with ethanol gradient, and infiltrated with epoxy

Do not freeze

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