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Oncotarget 6 , 2439324403

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To make excitatory neurons or astrocytes sensitive to blue light, 1 h after plating (DIV 0 and DIV 2 for neurons and astrocytes, respectively) Ngn2 -hiPSCs or astrocytes were transduced with an adeno-associated virus (AAV) serotype 2 encoding Channelrhodopsin-2 (ChR2) (AAV2-hSyn-hChR2(H134R)-mCherry, UNC Vector Core) 74 and AAV serotype 2 encoding ChR2 (AAV-GFAP-hChR2(H134R)-mCherry, UNC Vector Core) 44,75 , respectively

The interactions within the TVS, immune cells, and ongoing neurogenic or neuroinflammatory responses play significant roles in migraine pathophysiology, potentially leading to the establishment of chronic maladaptive pain