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Paraffin-embedded spinal cords were serially sectioned at 5 m steps, mounted on slides, and processed for Nissl staining

Tumor cells change their characteristics throughout the process, which enable them to proliferate and migrate, invade surrounding tissue, intravasate through the basement membrane into blood or lymphatic vessels, survive during circulation through the blood or lymphatic system, stay at distant sites, extravasate into a new environment tissue, and proliferate by inducing angiogenesis [10,11,12]